Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein.
Identifieur interne : 005046 ( Main/Exploration ); précédent : 005045; suivant : 005047Suppression of SARS-CoV entry by peptides corresponding to heptad regions on spike glycoprotein.
Auteurs : Kehu Yuan [République populaire de Chine] ; Ling Yi ; Jian Chen ; Xiuxia Qu ; Tingting Qing ; Xi Rao ; Pengfei Jiang ; Jianhe Hu ; Zikai Xiong ; Yuchun Nie ; Xuanling Shi ; Wei Wang ; Chen Ling ; Xiaolei Yin ; Keqiang Fan ; Luhua Lai ; Mingxiao Ding ; Hongkui DengSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Dichroïsme circulaire, Données de séquences moléculaires, Dosage biologique, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Peptides (), Peptides (génétique), Peptides (métabolisme), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Structure secondaire des protéines, Séquence d'acides aminés, Virus du SRAS (métabolisme).
- MESH :
- génétique : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines recombinantes.
- métabolisme : Glycoprotéines membranaires, Peptides, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS.
- Dichroïsme circulaire, Données de séquences moléculaires, Dosage biologique, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Peptides, Protéines de l'enveloppe virale, Structure secondaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Biological Assay, Cell Line, Circular Dichroism, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Peptides (chemistry), Peptides (genetics), Peptides (metabolism), Protein Structure, Secondary, Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Peptides, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Peptides, Recombinant Proteins, Viral Envelope Proteins.
- chemical , metabolism : Membrane Glycoproteins, Peptides, Recombinant Proteins, Viral Envelope Proteins.
- metabolism : SARS Virus.
- Amino Acid Sequence, Biological Assay, Cell Line, Circular Dichroism, Humans, Molecular Sequence Data, Protein Structure, Secondary, Spike Glycoprotein, Coronavirus.
Abstract
Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of these viruses. SARS-CoV was also predicted to have HR1 and HR2 regions in the S2 protein. Based on this prediction, we designed 25 peptides and screened them using a HIV-luc/SARS pseudotyped virus assay. Two peptides, HR1-1 and HR2-18, were identified as potential inhibitors, with EC(50) values of 0.14 and 1.19microM, respectively. The inhibitory effects of these peptides were validated by the wild-type SARS-CoV assay. HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry.
DOI: 10.1016/j.bbrc.2004.05.046
PubMed: 15184046
Affiliations:
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Le document en format XML
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type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Yi, Ling" sort="Yi, Ling" uniqKey="Yi L" first="Ling" last="Yi">Ling Yi</name></author><author><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name></author><author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name></author><author><name sortKey="Qing, Tingting" sort="Qing, Tingting" uniqKey="Qing T" first="Tingting" last="Qing">Tingting Qing</name></author><author><name sortKey="Rao, Xi" sort="Rao, Xi" uniqKey="Rao X" first="Xi" last="Rao">Xi Rao</name></author><author><name sortKey="Jiang, Pengfei" sort="Jiang, Pengfei" uniqKey="Jiang P" first="Pengfei" last="Jiang">Pengfei Jiang</name></author><author><name sortKey="Hu, Jianhe" sort="Hu, Jianhe" uniqKey="Hu J" first="Jianhe" last="Hu">Jianhe Hu</name></author><author><name sortKey="Xiong, Zikai" sort="Xiong, Zikai" uniqKey="Xiong Z" first="Zikai" last="Xiong">Zikai Xiong</name></author><author><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name></author><author><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name></author><author><name sortKey="Ling, Chen" sort="Ling, Chen" uniqKey="Ling C" first="Chen" last="Ling">Chen Ling</name></author><author><name sortKey="Yin, Xiaolei" sort="Yin, Xiaolei" uniqKey="Yin X" first="Xiaolei" last="Yin">Xiaolei Yin</name></author><author><name sortKey="Fan, Keqiang" sort="Fan, Keqiang" uniqKey="Fan K" first="Keqiang" last="Fan">Keqiang Fan</name></author><author><name sortKey="Lai, Luhua" sort="Lai, Luhua" uniqKey="Lai L" first="Luhua" last="Lai">Luhua Lai</name></author><author><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name></author><author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Biological Assay</term><term>Cell Line</term><term>Circular Dichroism</term><term>Humans</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Molecular Sequence Data</term><term>Peptides (chemistry)</term><term>Peptides (genetics)</term><term>Peptides (metabolism)</term><term>Protein Structure, Secondary</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Dichroïsme circulaire</term><term>Données de séquences moléculaires</term><term>Dosage biologique</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptides ()</term><term>Peptides (génétique)</term><term>Peptides (métabolisme)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptides</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptides</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptides</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Peptides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Peptides</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Biological Assay</term><term>Cell Line</term><term>Circular Dichroism</term><term>Humans</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dichroïsme circulaire</term><term>Données de séquences moléculaires</term><term>Dosage biologique</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptides</term><term>Protéines de l'enveloppe virale</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Heptad repeat regions (HR1 and HR2) are highly conserved sequences located in the glycoproteins of enveloped viruses. They form a six-helix bundle structure and are important in the process of virus fusion. Peptides derived from the HR regions of some viruses have been shown to inhibit the entry of these viruses. SARS-CoV was also predicted to have HR1 and HR2 regions in the S2 protein. Based on this prediction, we designed 25 peptides and screened them using a HIV-luc/SARS pseudotyped virus assay. Two peptides, HR1-1 and HR2-18, were identified as potential inhibitors, with EC(50) values of 0.14 and 1.19microM, respectively. The inhibitory effects of these peptides were validated by the wild-type SARS-CoV assay. HR1-1 and HR2-18 can serve as functional probes for dissecting the fusion mechanism of SARS-CoV and also provide the potential of further identifying potent inhibitors for SARS-CoV entry.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><noCountry><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name><name sortKey="Fan, Keqiang" sort="Fan, Keqiang" uniqKey="Fan K" first="Keqiang" last="Fan">Keqiang Fan</name><name sortKey="Hu, Jianhe" sort="Hu, Jianhe" uniqKey="Hu J" first="Jianhe" last="Hu">Jianhe Hu</name><name sortKey="Jiang, Pengfei" sort="Jiang, Pengfei" uniqKey="Jiang P" first="Pengfei" last="Jiang">Pengfei Jiang</name><name sortKey="Lai, Luhua" sort="Lai, Luhua" uniqKey="Lai L" first="Luhua" last="Lai">Luhua Lai</name><name sortKey="Ling, Chen" sort="Ling, Chen" uniqKey="Ling C" first="Chen" last="Ling">Chen Ling</name><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name><name sortKey="Qing, Tingting" sort="Qing, Tingting" uniqKey="Qing T" first="Tingting" last="Qing">Tingting Qing</name><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name><name sortKey="Rao, Xi" sort="Rao, Xi" uniqKey="Rao X" first="Xi" last="Rao">Xi Rao</name><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Xiong, Zikai" sort="Xiong, Zikai" uniqKey="Xiong Z" first="Zikai" last="Xiong">Zikai Xiong</name><name sortKey="Yi, Ling" sort="Yi, Ling" uniqKey="Yi L" first="Ling" last="Yi">Ling Yi</name><name sortKey="Yin, Xiaolei" sort="Yin, Xiaolei" uniqKey="Yin X" first="Xiaolei" last="Yin">Xiaolei Yin</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yuan, Kehu" sort="Yuan, Kehu" uniqKey="Yuan K" first="Kehu" last="Yuan">Kehu Yuan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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